Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.2.1.25 extracted from

  • Altenschmidt, U.; Oswald, B.; Steiner, E.; Herrmann, H.; Fuchs, G.
    New aerobic benzoate oxidation pathway via benzoyl-coenzyme A and 3-hydroxybenzoyl-coenzyme A in a denitrifying Pseudomonas sp. (1993), J. Bacteriol., 175, 4851-4858.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.2.1.25

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 benzoate anaerobically induced enzyme Pseudomonas sp.
0.011
-
 benzoate benzoate, , aerobically induced enzyme Pseudomonas sp.
0.089
-
 CoA anaerobically induced enzyme Pseudomonas sp.
0.09
-
 2-Fluorobenzoate aerobically induced enzyme Pseudomonas sp.
0.1
-
 CoASH 2-fluorobenzoate, , anaerobically induced enzyme Pseudomonas sp.
0.105
-
 CoA aerobically induced enzyme Pseudomonas sp.
0.125
-
 ATP anaerobically induced enzyme Pseudomonas sp.
0.277
-
 ATP aerobically induced enzyme Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
 2 * 54000, SDS-PAGE, anaerobically induced enzyme Pseudomonas sp.
56000
-
 2 * 56000, SDS-PAGE, aerobically induced enzyme Pseudomonas sp.
120000
-
 gel filtration, anaerobically induced enzyme Pseudomonas sp.
130000
-
 gel filtration, aerobically induced enzyme Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp.
-
 an anaerobically induced benzoate-CoA ligase and an aerobically induced benzoate-CoA ligase with different properties
-
Pseudomonas sp. KB740
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
73
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-aminobenzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + 2-aminobenzoyl-CoA
-
 ?
ATP + 2-fluorobenzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
ATP + 2-fluorobenzoate + CoA
-
 Pseudomonas sp. KB740 AMP + diphosphate + 2-fluorobenzoyl-CoA
-
 ?
ATP + 2-methylbenzoate + CoA no activity with anaerobically induced enzyme, 15% of the activity relative to benzoate with the aerobically induced enzyme Pseudomonas sp. AMP + diphosphate + 2-methylbenzoyl-CoA
-
 ?
ATP + 3-fluorobenzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + 3-fluorobenzoyl-CoA
-
 ?
ATP + 4-fluorobenzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + 4-fluorobenzoyl-CoA
-
 ?
ATP + benzoate + CoA
-
 Pseudomonas sp. AMP + diphosphate + benzoyl-CoA
-
 ?
ATP + benzoate + CoA
-
 Pseudomonas sp. KB740 AMP + diphosphate + benzoyl-CoA
-
 ?
additional information not 3-hydroxybenzoate Pseudomonas sp. ?
-
 ?
additional information not 3-hydroxybenzoate Pseudomonas sp. KB740 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 54000, SDS-PAGE, anaerobically induced enzyme Pseudomonas sp.
dimer 2 * 56000, SDS-PAGE, aerobically induced enzyme Pseudomonas sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 aerobically induced enzyme Pseudomonas sp.
9.3
-
 anaerobically induced enzyme Pseudomonas sp.